Biacore t200 upgrade kit 28977954 biacore t200 gxp upgrade kit 28977964 biacore t200 software v3. Classroom courses practical and theoretical courses are available on the use of biacore systems. Biacore t200 works on the principle of surface plasmon resonance spr, surface plasmon resonance spr allows realtime, labelfree detection of biomolecular interactions. Text format export to biacore biaevaluation and other thirdparty software. Biacore t200 gxp upgrade kit 28977964 biacore t200 software v2. This edition of the biacore x100 handbook applies to systems running software version 2.
Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. Our high quality bioanalytical services using the powerful technique of surface plasmon resonance spr, biacore provides labelfree, real time analysis of any pair of interacting molecules including small molecule drugs binding to their target proteins in more than 15 years biaffin acquired expert knowledge in application of biacorespr technology and has proven to. Label free interaction analysis ge healthcare fig 1. It works for the control software with the biacore 20003000. Biacore t200 cytiva, formerly ge healthcare life sciences. Determination of adenovirus concentration using biacore. Compare the reichert sr7500 dual channel system to the.
Cross new frontiers in labelfree interaction analysis with biacore t200 28979412 data file. Biacore t200 getting started guide integrated structural biology. Data file 29050011 aa labelfree interaction analysis biacore t200 software v2. Safety information biacore x100 instrument contains mains voltage and handles liquids that may be hazardous. Cfca was performed using a biacore t200 ge healthcare to determine the active concentration of the mabs. Full text labelfree interaction analysis as a tool to demonstrate. Is a noninvasive label free technology based on surface plasmon resonance spr principle. With a single click, the compact designed equipment offers. The software provides dedicated support for kinetics, affinity. Data file 29050011 aa label free interaction analysis biacore t200 software v2. Biacore t200 software handbook 28976878 edition aa. For more information on spr technology and biacore s products.
The system delivers high quality kinetic, affinity, concentration. The interaction partner attached to the surface is called the ligand figure 11. Biacore t200 biacore t200 fig 1 is a versatile, labelfree system for detailed studies of biomolecular interactions, from early research to drug discovery and development, and on to quality control qc. Background information sensor surface properties 10 biacore t200 getting started 28984098 edition ab there are three major steps in a biacore assay. Cross new frontiers in label free interaction analysis with biacore t200 28979412 data file. Undock the maintenance chip and store it in an empty 50 ml tube to keep it free of. Labelfree interaction analysis base your decisions on the best biacore systems from ge healthcare for over 15 years, biacore systems have enabled comprehensive, labelfree characterization of binding events, giving unique insights into how proteins and other biomolecules interact with each other, and helping scientists. Biacore t200 software handbook, the instruction manual for biacore. Ge biacore t200 getting started guide ligand analyte. Epitope mapping immunogenicity concentration analysis parallel line analysis. Biacore t from ge healthcare is a high performance system for realtime biomolecular interaction analysis, using surface. Biacore x100 is intended for research use only and should not be used for diagnostic purposes in any clinical or in vitro procedures. Biacore t200 for high sensitivity, labelfree interaction analysis. Data file 28979415 ab label free interaction analysis biacore t200 biacore t200 fig 1 is a versatile, label free system for detailed studies of biomolecular interactions, from early research to drug discovery and development, and on to qc.
Biacore t200 is a system for realtime labelfree analysis of molecular. The biacore t200 is an instrument for surface plasmon resonance. The system delivers high quality kinetic, affinity, concentration, specificity, selectivity, comparability, and thermodynamic. Schematic illustration showing the flow paths during loop injection. Biacore assay handbook 29019400 edition aa 5 introduction 1 1 introduction 1. Biacore assay handbook ge healthcare life sciences. Biacore t200 instrument handbook describes the instrumentation in the biacore t200 system, with instructions for operation, maintenance and troubleshooting.
Surface plasmon resonancebased determination of adenovirus concentration on biacore t200. The analyte is injected over the sensor chip surface and the interaction between the analyte and the. Ge healthcare labelfree interaction analysis biacore t200. Advances in labelfree protein interaction technology by biacore result in continuously. The biacore t200 is an instrument for surface plasmon.
Biacore t200 getting started 28984098 edition ab 5 biacore t200 getting started biacore t200 getting started introduction this getting started handbook is designed as a selfstudy guide to introduce you to the basic operations of biacoretm t200, biacore t200 control software and biacore t200 evaluation software. Turn your pdf or hard copy worksheet into an editable digital. So i am using biacore t200 and want to perform a kinetics analysis on a small molecule that is bound to my protein of interest. Advances in label free protein interaction technology by biacore result in continuously. Biacore spr systems label free, real time data for deeper insights.
Spr occurs when polarized light strikes an electrically conducting surface at the interface between two media. Determination of adenovirus concentration using biacore t200 this application note demonstrates the performance of two assays for determination of adenovirus concentration based on surface plasmon resonance spr. Streamlined workflow oneclick integration with tracedrawer software for downstream data evaluation. From biacore t100 to biacore t200 2 5 6 8 10 lod 0. Please click here for individual platformresource costings. Principles of immunogenicity assessment using biacore t200. Data file, ka9454290819df labelfree interaction analysis biacore t200 gxp package for systems used in regulated applications, biacore t200 gxp package facilitates compliance with worldwide regulatory. B determined over the same sensor surface, in order to calculate the free b concentrations in the samples. Biacore t200, ge healthcare, 28979415, edition ab 20. Biacore assay handbook ge healthcare life sciences pages 1. Data file 28979415 ab labelfree interaction analysis biacore t200 biacore t200 fig 1 is a versatile, labelfree system for detailed studies of biomolecular interactions, from early research to drug discovery and development, and on to qc.
The underlying physics of the principles of spr are complicated. Reichert sr7500dc biacore x100 biacore t200 number of channels 22 4 injection of lysates, cells, particulates vyes no no diy fluidics vyes no no autosampler capacity vmax. Biacore 4000 offers a powerful solution for largescale label free interaction analysis, delivering high quality data without compromising on throughput. In one assay, virus particles were allowed to bind via the fiber protein spikes extruding on the virus capsid to recombinant. The system is intended for research and development. The partner attached to the surface is called the ligand. Kinetic interactions are modelled using a two compartment model where transport of analyte to the sensor surface and binding at the sensor. Data file 29007927 aa biacore labelfree interaction analysis sensor chip nta nta reagent kit sensor chip nta and nta reagent kit are designed for capture of histidinetagged recombinant proteins in biacore systems. Biacore t200, biacore t200 control software version 2. The new t200 control software is newly designed and not compatible with biamethodwriter. The following terms are used in the context of work with biacore systems see figure 11.
The symbols o and represent open and closed valves respectively. Both samples and reagents are taken from standard microplates with no need for special vials. This eight needle highsensitivity surface plasmon resonance spr system rapidly provides kinetics and. Ge healthcare labelfree interaction analysis biacore 8k. To find out about other training opportunities, contact your local.
Legal information regarding software licensing and details for acquiring additional licenses. Your advantages of using biaffins biacore spr services. Biacore t100 instrument handbook br100647 edition ad 7 introduction 1 1 introduction 1. You can use biacore t200 instrument, on which you can include the solvent correction process in your experiment, using the calibration solutions 18 in the attached file. Cross new frontiers in label free interaction analysis with biacore t200, ge healthcare, 28979412, edition aa 2010 data file. Biacore systems monitor the interaction between two molecules, of which one is attached to the sensor surface and the other is free in solution. The biamethodwriter is a program to write the biacore automation methods in a more interactive way. Biacore maintenance kits are convenient kits for proper instrument maintenance. Overview of biacore systems and their applications. The process by which the ligand is attached to the sensor chip surface. Biacore t200 spr platform biacore t200 analyzes and characterizes adas and molecular interactions related to kinetics, specificity, and concentration. One platform for comprehensive characterization of molecular interactions in terms of kinetics, affinity, specificity, comparability, concentration, immunogenicity, and thermodynamics.
In biacore x100 and biacore t200 evaluation software ge healthcare, uppsala, sweden, numerical integration of rate equations, coupled to an algorithm that minimize the difference between observed and calculated data, is used. Manual run provides interactive control of the instrument operation, executing. Is a noninvasive labelfree technology based on surface plasmon resonance spr principle. The following terms are used in the context of biacore based assays. Biacore t200 is a versatile system for highquality characterization of molecular interactions, from ions to viruses. Data file 29050011 aa labelfree interaction analysis. Enhanced performance increased dynamic range for biacore t100t200 instruments. Warning biacore t200 instrument contains mains voltage of up to 265 v ac. In a typical spr experiment, one molecule the ligand is immobilized to a sensor chip and. Reacts to changes in the concentration of molecules. Sensitivity for confident analysis of the most challenging interactions. Biacore t200 getting started guide spr platform last modified. For more information on spr technology and biacores products.
Capture is an attractive alternative for protein attachment since physiological conditions can be used during the procedure. Evaluation of calibrationfree concentration analysis. Sensor chip cm5 cytiva, formerly ge healthcare life sciences. Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Data file 29050011 aa labelfree interaction analysis biacore. Oct 12, 2015 labelfree assays for highthroughput monoclonal antibody characterization. Any functionality that is added in optional addon modules is described in separate documentation. Oct 12, 2015 this video show you how to startup and prepare a biacore t200 system for use. Latest software updates and software notes software upgrade packages and additional licences software wizard templates to help speed up assay optimization and routine analyses. Ge biacore t200 getting started guide introduction the biacore t200 is an instrument for surface plasmon resonance spr, an optical technique that measures changes in refractive index near a metal surface over time. Well equipped with a number of different spr biosensors 5x biacore t200, 1x biacore 2000 we can ensure a rapid and individual analysis of your samples following work plans tailored to your specific needs.